Test tube baby laboratory additional treatment: promising but not always helpful

1. Time-lapse imaging (TLS) embryos develop in the incubator, undergoing fertilization to cleavage stage, and some cases to blastocyst stage. The embryologist examines the developing embryo in order to select the embryo most likely to be implanted and develop into a fetus. Traditionally, the embryos were removed from the controlled incubator environment and briefly placed under a microscope for examination by an embryologist. TLS allows embryologists to monitor developing embryos without removing them from the incubator. Based on morphology, early mitotic time and synchronization, and abnormal splitting patterns, these are morphological dynamic parameters, so the best embryos are selected for transplantation. The potential advantages of TLS include: the availability of detailed digital images of developing embryos, which can be used to edit the time-lapse sequence of embryo development; providing an undisturbed culture environment for embryos, avoiding embryos being exposed to mechanical interference and Changes in temperature, pH, humidity, and gas composition; Embryo selection software can be used to help embryologists select the best embryo transfer based on morphological kinetic parameters and inclusion and exclusion criteria through complex algorithms. Possible disadvantages include increased osmotic pressure of cultured droplets, because some TLS cannot be humidified, and a cycle of $1,000 is borne by patients, although not all reproductive clinics charge patients. A 2019 Cochrane system review included 9 RCTS (2955 women). Compared with conventional culture, it is unclear whether the time-lapse imaging plus algorithm is different in live birth rate. Overall, the review concluded that there is insufficient sufficient quality evidence (therewasinsufficientgoodqualityevidenceofdifferencesinlivebirth…) to show the choice between conventional culture and TLS (with or without embryo selection software) between live births or continuous pregnancy, miscarriage, stillbirth or clinical pregnancy There is a difference. The evidence is of low or very low quality (theevidence is low or very low quality). The British Human Fertilization and Embryology Bureau (HFEA) believes that the evidence is contradictory to TLS, and there is indeed insufficient evidence to show that time-lapse imaging improves the birth rate.